نتایج جستجو برای: restriction fragment length

تعداد نتایج: 412621  

Journal: :jundishapur journal of microbiology 0
afsaneh moaddeb department of pathobiology, school of veterinary medicine, shiraz university, shiraz, ir iran mohammad reza fattahi gastroentrohepatology research center, shiraz university of medical sciences, shiraz, ir iran roya firouzi department of pathobiology, school of veterinary medicine, shiraz university, shiraz, ir iran abdollah derakhshandeh department of pathobiology, school of veterinary medicine, shiraz university, shiraz, ir iran shohreh farshad clinical microbiology research center, shiraz university of medical sciences, shiraz, ir iran; clinical microbiology research center, shiraz university of medical sciences, p. o. box: 7193711351, shiraz, ir iran. tel: +98-7136474304; +98-9173173501, fax: +98-7136474303

conclusions using the pcr-rflp method, three distinctive h. pylori caga genotypes were detected in antral biopsies. genotype i, which was predominant among the isolates, was significantly associated with gastritis. however, the data showed that caga genotype iii may play a role in duodenitis and duodenal ulcers in patients infected with h. pylori. patients and methods a total of 161 gastric bio...

Journal: :jundishapur journal of microbiology 0
shohreh farshad professor alborzi clinical microbiology research center, nemazi hospital, shiraz university of medical sciences, shiraz, ir iran; corresponding author: shohreh farshad, professor alborzi clinical microbiology research center, nemazi hospital, shiraz university of medical sciences, p. o. box: 7193711351, shiraz, ir iran. tel: +98-7116474304, fax: +98-7116474303, e-mail: reza ranjbar molecular biology research center, baqiyatallah university of medical sciences, tehran, ir iran marziyeh hosseini professor alborzi clinical microbiology research center, nemazi hospital, shiraz university of medical sciences, shiraz, ir iran

conclusions the results revealed that the strains under study could be epidemically related. it seems that an alternative subtyping method is needed to study the relationship among clinical s. sonnei strains and their transmission. here, we reported for the first time, two strains of s. sonnei with a different pcr-rflp pattern for ipah gene. results all shigella isolates were positive for both ...

Journal: :Applied and environmental microbiology 2007
Noah Fierer

Blackwood et al. (1) assert that terminal restriction fragment length polymorphism (T-RFLP) data cannot be used to estimate the species level diversity of bacterial communities. While I do not argue with their analytical techniques, nor the interpretation of their results, their conclusion is essentially moot as T-RFLP techniques are rarely used (nor should they be used) to study the diversity ...

Journal: :Journal of clinical microbiology 2008
Tomasz Hauschild Srdjan Stepanovic

A PCR-restriction fragment length polymorphism (RFLP) analysis method that analyzes a part of the dnaJ gene was designed for the rapid and accurate identification of Staphylococcus spp. XapI or Bsp143I digestion of the PCR-generated products rendered distinctive RFLP patterns that allowed 41 reference species and subspecies to be identified with a high degree of specificity. The novel method wa...

Journal: :hepatitis monthly 0
rim ouneissa laboratory of clinical virology, institute pasteur de tunis, tunis, tunisia olfa bahri laboratory of clinical virology, institute pasteur de tunis, tunis, tunisia; laboratory of clinical virology, institute pasteur de tunis, tunis, bp 1002, tunisia. tel: +216-98334999, fax: +216-71791833 ahlem ben yahia laboratory of clinical virology, institute pasteur de tunis, tunis, tunisia henda touzi laboratory of clinical virology, institute pasteur de tunis, tunis, tunisia mohamed msaddak azouz department of gastroenterology, hospital of tahar maamouri, nabeul, tunisia nabyl ben mami department of gastroenterology, hospital la rabta, tunis, tunisia

conclusions several previous studies used pcr-rflp to genotype hbv; however, we showed the high risk to obtain atypical profiles, especially in advanced stages of chronic infection, with as results difficulties to genotype the virus. these profiles resulted from the accumulation of mutations during natural course of infection resulting in a modification in restriction sites for enzymes. so, we ...

ژورنال: :مجله دانشکده پزشکی اصفهان 0
محمد بهمئی دانشجوی کارشناسی ارشد، گروه انگل و قارچ شناسی، دانشکده ی پزشکی و کمیته ی تحقیقات دانشجویی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران پروین دهقان استادیار، گروه انگل و قارچ شناسی، دانشکده ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران رسول محمدی استادیار، گروه انگل و قارچ شناسی، دانشکده ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران جواهر چعباوی زاده استادیار، گروه انگل و قارچ شناسی، دانشکده ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران بهزاد مهکی استادیار، گروه آمار زیستی، دانشکده ی بهداشت، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

مقدمه: candidiasis منتشره، دیابت، حاملگی، مصرف بی رویه ی آنتی بیوتیک های وسیع الطیف، عوامل مستعد کننده ی candidiasis دستگاه ادراری می باشند. شیوع عفونت با گونه های غیر candida albicans رو به افزایش است. هدف از انجام این مطالعه، بررسی میزان شیوع گونه های candida مسبب کاندیدوری در شهر اصفهان با استفاده از روش polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) بود. روش ه...

Journal: :iranian journal of public health 0
sf tee py tang hc loh

background: molecular components of the dopamine receptor (drd3) play an important role in the pathophysiology of schizophrenia (scz). previous studies have demonstrated an association between the drd3 ser9gly polymorphism and scz but the results have been inconclusive. method: in this study, we investigated this controversial association between the ser9gly (a/g) polymorphism and scz using mal...

Journal: :jundishapur journal of microbiology 0
zeinab ghasemi department of medical parasitology and mycology, school of public health, national institute of health research, tehran university of medical sciences, tehran, ir iran seyed jamale hashemi department of medical parasitology and mycology, school of public health, national institute of health research, tehran university of medical sciences, tehran, ir iran; department of medical parasitology and mycology, school of public health, national institute of health research, tehran university of medical sciences, tehran, ir iran. tel: +98-21009141 sassan rezaei department of medical parasitology and mycology, school of public health, national institute of health research, tehran university of medical sciences, tehran, ir iran parivash kordbache department of medical parasitology and mycology, school of public health, national institute of health research, tehran university of medical sciences, tehran, ir iran mojtaba khosravi department of pathobiology, faculty of veterinary medicine, amol university of special modern technologies, amol, iran hossein mortazavi department of dermatology, school of medicine, razi hospital, tehran university of medical sciences, tehran, ir iran

conclusions the results of the present study, compared to the previous studies, showed a significantly lower prevalence of cutaneous candidiasis. among candida species, c. albicans was still the most common infectious agent isolated from clinical samples. shifting toward non-albicans candida species was not confirmed in this study. in addition, our study revealed that there is a direct correlat...

Journal: :journal of medical bacteriology 0
seyyedsaeed eshraghi section microbiology, department pathobiology, school of public health, tehran university of medical sciences, tehran, ir iran.

identification  of clinically  significant  nocardia  species  is essential  for the definitive diagnosis, predict antimicrobial susceptibility, epidemiological purposes,  and  for  an  effective  treatment.   conventional   identification   of nocardia species in routine medical laboratories which is based on phenotypic (cellular morphology, colonial characteristics), biochemical and enzymatic...

Journal: :iranian red crescent medical journal 0
ali mohammad foroughmand faculty of science, department of genetics, shahid chamran university of ahvaz, ahvaz, ir iran zahra shahbazi faculty of science, department of genetics, shahid chamran university of ahvaz, ahvaz, ir iran; faculty of science, department of genetics, shahid chamran university of ahvaz, ahvaz, ir iran. tel: +98-9380889172 hamid galehdari faculty of science, department of genetics, shahid chamran university of ahvaz, ahvaz, ir iran mahdi purmahdi borujeni faculty of veterinary medicine, department of food hygiene, shahid chamran university of ahvaz, ahvaz, ir iran parvane dinarvand faculty of science, department of genetics, shahid chamran university of ahvaz, ahvaz, ir iran khadije golabgirkhademi faculty of science, department of genetics, shahid chamran university of ahvaz, ahvaz, ir iran

background coronary artery disease (cad) is the most common cause of death worldwide. mef2a directly regulates target genes in the process of muscle development. this gene product is a transcription factor. mef2a protein in homodimer or heterodimer forms binds to a/t-rich cis elements with conserved sequence in promoter, regulator, and enhancer of many genes, which are determining in evolution ...

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